Journal: Frontiers in Endocrinology

Article Title: Restoring Impaired Fertility Through Diet: Observations of Switching From High-Fat Diet During Puberty to Normal Diet in Adulthood Among Obese Male Mice

doi: 10.3389/fendo.2022.839034

Figure Lengend Snippet: The expression of synthetic testosterone and spermatogenic enzymes induced by a high-fat diet during sexual development. (A) The bands of Western blotting analysis of StAR, WT-1, DAZL in mice testes. Testis lysates (40 µg/well) were loaded onto gel and reacted with primary and later secondary antibodies; GAPDH was set as loading control. The panel (B–D) is a densitometric histogram of protein bands and results were expressed as ratio of corresponding protein to GAPDH (n = 3 for each group). Results are presented as mean ± SEM. #p < 0.05, ##p < 0.01 vs the NN group. All of the above panels are representative of 3 independent experiments.

Article Snippet: The membranes were blocked with 5% non-fat dry milk in Tris-buffered saline (TBS) for 1 h, and then incubated with specific primary antibodies StAR (1:1000, 8449S, CST), WT-1 (1:1000, 83535S, CST) and DAZL (1:1000, 12633-1-AP, Proteintech) overnight at 4°C.

Techniques: Expressing, Western Blot, Control

Journal: All Life

Article Title: HMGCS1 Promotes male differentiation of chicken embryos by regulating the generate of cholesterol

doi: 10.1080/26895293.2021.1936207

Figure Lengend Snippet: Figure 4. HMGCS1 promotes male differentiation in chicken embryos. (A) The expression levels of some sex-related genes (HMGCS1, CYP19A1,DMRT1,SOX9)weredetectedwhentheembryonicbodyinterferedwithandoverexpressedHMGCS1anddevelopedto4.5days. F is for female and M is for male. The mean ± sd. is shown; n = 3 independent experiments. P by t-test. ∗P < 0.05. (B) The expression levels of some sex-related genes (HMGCS1, CYP19A1, DMRT1, SOX9) were detected when the chicken embryos interfered and overex- pressed HMGCS1 and developed to 18.5 days. The mean ± sd. is shown; n = 3 independent experiments. P by t-test. ∗P < .05. (C) In the detection of testicular morphology after HMGCS1 overexpression and interference. The brown color is produced by immunofluorescence labeling of germ cells with DAZL antibody, the arrows indicate convoluted seminiferous tubule. (D) Quantification of figure C, the Y-axis represents the ratio of convoluted tubule area to total testicular cross-sectional area.

Article Snippet: The sections were labeled with DAZL antibody according to the instructions of the SABC immunohistochemical staining kit (Boster, China), and finally the DAB color development kit (Boster, China) was used for color development.

Techniques: Expressing, Over Expression, Produced, Labeling